Coding

Part:BBa_K5048069:Design

Designed by: Ziyan Peng   Group: iGEM24_WHU-China   (2024-09-24)


Neae-HSP60-FLAG


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2006
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2347
    Illegal AgeI site found at 2502
    Illegal AgeI site found at 2980
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1244


Design Notes

We constructed this part by restriction endonuclease cleavage and T4 ligation. During the process of design, we had to consider the enzyme and the cleavage site we needed to use. When inserted into the pET-28a backbone, this part is included in Adhesion Construction(Fig 1).

Fig 1 The genetic circuit respectively for pET-28a-[Neae-LAP] (left)

Source

See our parts: BBa_K5048047, BBa_K5048060


References